Current Advances in the Functional Genes of Edible and Medicinal Fungi: Research Techniques, Functional Analysis, and Prospects
Round 1
Reviewer 1 Report
The manuscript reviews the main techniques used for genetic engineering of edible fungi as well as the functional genes that can be targeted for the improvement of industrial processes. Each technique is clearly described and advances in functional characterization of genes of several edible fungi are included. The discussion on mating genes is key since it is very specific to edible fungi
General
At a first glance it seems that authors focused on wild edible fungi or those grown by solid fermentation. Explaining more about advances in edible fungi grown by liquid fermentation and adding the terms “precision fermentation “ as well would certainly improve the impact of the article
Line 253. IS there any example of the use of iRNA for process improvement in the industry? How is it or would it be applied in a large scale?
LINES 954-958. Please cite references
Introduction
Line 36. What do you mean with living substances?
Conclusion
Authors describe the main issues that are now delaying research in fungal genetic engineering
I would add false taxonomic identities assigned to fungal strains in scientific articles and public “omic” databases
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments to the Authors
The authors have written the review article well; it contains authentic scientific information and explores gene-related facets. However, the review does not properly organize the presentation of the article. Authors will bring about articles directly relevant to the issue, which is highly valued and engaging for readers. This review offers a thorough overview of the advantages and disadvantages of different functional genes. clearly provided a brief description of the advantages along with relevant details. The paper cites 59 occurrences of Ganoderma and 45 occurrences of Cordyceps. The author believes that these species are classified as edible fungi. The author must stick to the title or consider revising it.
Comments to the Authors
Authors should contemplate various effective techniques to tackle the subject matter when dealing with a broad title. These strategies involve incorporating knowledge about agaricomycete gene function, employing advanced functional-omics techniques in recent studies, utilizing omics research to reveal gene function and important biological processes, utilizing CRISPR-Cas9 assisted techniques for functional gene manipulation, investigating gene function and functional hypotheses, and conducting experiments involving gene knockout, knockdown, tuning, editing, and overexpression. In addition, techniques such as photomorphogenesis have been created to enhance the production of metabolites and increase food quality.
The content found in lines 44 to 64 of the introduction is irrelevant to the current topic. The writers review the mushroom industry, focusing on production, substrate, and sustainable industrial practices, which are crucial aspects of the subject matter. Nevertheless, these statements do not appear directly relevant to the subject matter. Please revise the information to ensure it's pertinent to the topic.
Figure 1. The accuracy of the illustration visuals should be verified using empirical data and enhanced in the image section.
Please confirm the accuracy of the citation at line 658, specifically reference number 140, which corresponds to the research conducted by Lyu et al. in 2021.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
