Variations in Morpho-Cultural Characteristics and Pathogenicity of Fusarium moniliforme of Bakanae Disease of Rice and Evaluation of In Vitro Growth Suppression Potential of Some Bioagents
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Site
2.2. Isolation, Purification, and Identification of the Pathogen
2.3. Cultural and Morphologic
2.4. Pathogenicity Test
2.5. In Vitro Evaluation of Bioagents to Suppress the Growth of the Target Pathogen of Bakanae Disease of Rice
2.5.1. Multiplication of Bioagents
2.5.2. In Vitro Growth-Suppressing Ability of Bioagents against Fusarium moniliforme
2.6. Disease Assessment
2.7. Statistical Analyses
3. Results
3.1. Cultural and Morphological Variation in Fusarium spp.
3.2. Evaluation of Pathogenic Variation in Different Isolates of Fusarium spp.
3.3. In Vitro Growth Suppression of Fusarium moniliforme by Bacillus subtilis
3.4. In Vitro Growth Suppression of Fusarium moniliforme by Pseudomonas fluorescens
3.5. In Vitro Growth Suppression of Fusarium moniliforme by Achromobacter spp.
3.6. In Vitro Growth Suppression of Fusarium moniliforme by Trichoderma spp.
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sl. No. | Isolate Name | Season | Host | District | Village | AEZ |
---|---|---|---|---|---|---|
1 | FM3 | Aus | BRRI dhan 27 | Mymensingh | Babuakhali | AEZ 9 |
2 | FM4 | Aus | BRRI dhan 27 | Mymensingh | Sutiakhali | AEZ 9 |
3 | FM5 | Aus | BRRI dhan 27 | Mymensingh | Beltuli | AEZ 9 |
4 | FM6 | Aus | BRRI dhan 27 | Mymensingh | Kurutoli | AEZ 9 |
5 | FM7 | Aman | BRRI dhan 49 | Mymensingh | BoroBabuakhali | AEZ 9 |
6 | FM8 | Aman | BRRI dhan 49 | Mymensingh | Curkhai | AEZ 9 |
7 | FM9 | Aman | BRRI dhan 49 | Mymensingh | Boro Bilar par | AEZ 9 |
8 | FM10 | Aman | BRRI dhan 34 | Mymensingh | Bagnabari | AEZ 9 |
9 | FM11 | Aman | BRRI dhan 34 | Mymensingh | Fulpori | AEZ 9 |
10 | FM12 | Aman | BRRI dhan 34 | Mymensingh | Chanakandi | AEZ 9 |
11 | FM13 | Aman | BRRI dhan 34 | Mymensingh | Vobakandi | AEZ 9 |
12 | FS16 | Boro | BRRI dhan 28 | Mymensingh | Bedkanda | AEZ 9 |
13 | FS18 | Boro | BRRI dhan 28 | Mymensingh | Kewatkhali | AEZ 9 |
14 | FS19 | Boro | BRRI dhan 28 | Sirajganj | LahiriMohanpur | AEZ 4 |
15 | FS20 | Boro | BRRI dhan 28 | Sirajganj | Caksa | AEZ 4 |
16 | FS21 | Boro | BRRI dhan 28 | Sirajganj | Kadapara | AEZ 4 |
17 | FS22 | Boro | BRRI dhan 28 | Sirajganj | Dohukula | AEZ 4 |
18 | FS23 | Boro | BRRI dhan 28 | Sirajganj | Boropangasi | AEZ 4 |
19 | FS24 | Boro | BRRI dhan 28 | Sirajganj | Srekola | AEZ 4 |
20 | FS25 | Boro | BRRI dhan 28 | Sirajganj | Dukuria | AEZ 4 |
21 | FS26 | Boro | BRRI dhan 28 | Dhaka | Salna | AEZ 4 |
22 | FS29 | Boro | BRRI dhan 28 | Dhaka | Salna | AEZ 4 |
23 | FS31 | Boro | BRRI dhan 28 | Dhaka | Gazipur | AEZ 28 |
24 | FS32 | Boro | BRRI dhan 29 | Dhaka | Gazipur | AEZ28 |
25 | FS34 | Boro | BRRI dhan 29 | Dhaka | BSMRAU | AEZ 28 |
26 | FD35 | Boro | BRRI dhan 29 | Dhaka | BSMRAU | AEZ 28 |
27 | FD41 | Boro | BRRI dhan 29 | Dhaka | SAU | AEZ 19 |
28 | FD45 | Boro | BRRI dhan 29 | Dhaka | SAU | AEZ 19 |
29 | FD47 | Boro | BRRI dhan 29 | Dhaka | Savar | AEZ8 |
30 | FD48 | Boro | BRRI dhan 29 | Dhaka | Savar | AEZ8 |
Rating Number | Reaction Description |
---|---|
0 | No disease symptoms |
1 | Normal growth but leaves beginning to show yellowish green color |
2 | Abnormal growth; elongated, thin, and yellowish-green leaves; seedlings also shorter or taller than normal |
3 | Abnormal growth; elongated, chlorotic, thin, and brownish leaves; seedlings also shorter or taller than normal |
4 | Seedlings with fungal mass on the surface of infected plants or dead plants |
Sl. No. | Isolates | Colony Features | Phialide | Chlamyd- ospore | |
---|---|---|---|---|---|
Colony Colors | Substrate Color | ||||
1 | FM3 | White orange to pale orange, grayish strip present | Orange to light orange, blackish strip | Monophialide and simple polyphialides | absent |
2 | FM4 | White orange to pale orange, grayish strip present | Orange to light orange, blackish strip | Monophialide and polyphialides | absent |
3 | FM5 | Pinkish white to grayish violet | Deep orange to pale orange | Simple and branch monophialide | absent |
4 | FM6 | Pinkish white to grayish violet | Deep orange to pale orange | Simple and branch monophialide | absent |
5 | FM7 | Pinkish white to pale orange | Light orange with grayish strip | Simple and branch monophialide | absent |
6 | FM8 | Pinkish white to pale orange | Deep orange to pale orange, grayish strip | Simple and branch monophialide | absent |
7 | FM9 | Pinkish white to grayish violet | Deep orange to grayish strip | Simple and branch monophialide | absent |
8 | FM10 | Pinkish white to grayish orange | Light orange to grayish strip | Simple and branch monophialide | absent |
9 | FM11 | White orange to pinkish white | Light orange with grayish strip | Monophialide and simple polyphialides | absent |
10 | FM12 | Pinkish white with gray strip | Light orange with grayish strip | Simple and branch monophialide | absent |
11 | FM13 | White orange to grayish violet | Light orange with grayish strip | Monophialide and simple polyphialides | absent |
12 | FS16 | Grayish white to gray | Deep orange, black in center | Monophialide and simple polyphialides | absent |
13 | FS18 | Pinkish white to grayish violet | Pale orange, deep orange in center | Simple and branches monophialide | absent |
14 | FS19 | White to grayish white, gray strip | Solid orange | Monophialide and simple polyphialides | absent |
15 | FS20 | Light orange with grayish strip | Light orange with grayish strip | Monophialide and simple polyphialides | absent |
16 | FS21 | Pinkish white to light brown | Deep and light orange patch | Monophialide and simple polyphialides | absent |
17 | FS22 | Pinkish white | Deep orange to dark black in center | Monophialide and polyphialides | absent |
18 | FS23 | Pale orange to grayish violet | Light orange at periphery and deep orange on center | Simple and branch monophialide | absent |
19 | FS24 | Pinkish white to grayish black, striped | Pale white to pale orange | Monophialide and polyphialides | absent |
20 | FS25 | Pale orange to grayish violet | Dark orange to dark gray | Monophialide and polyphialides | absent |
21 | FS26 | Pinkish white to pale orange | Pale orange | Monophialide and simple polyphialides | absent |
22 | FS29 | Pinkish white, pale orange in center | Dark orange and grayish strip | Monophialide and polyphialides | absent |
23 | FS31 | Pale orange to violet, striped | Dark orange and grayish strip | Monophialide and polyphialides | absent |
24 | FS32 | White, no pigmentation | Faded white | Monophialide and polyphialides | absent |
25 | FS34 | Orange-white to grayish violet | Pale orange to pale white | Monophialide and simple polyphialides | absent |
26 | FD35 | Pinkish white to pale orange | Deep orange on periphery, light in center | Monophialide and simple polyphialides | absent |
27 | FD41 | Pale orange to pinkish white | Deep orange on periphery, light in center | Simple and branch monophialide | absent |
28 | FD45 | Pinkish white and pale orange strip | Deep orange on periphery, light in center | Monophialide and polyphialides | absent |
29 | FD47 | Grayish violet | Pale orange to grayish violet | Monophialide and simple polyphialides | absent |
30 | FD48 | Pale orange to pinkish white | Deep orange on periphery, light in center | Monophialide and simple polyphialides | absent |
Sl. No. | Isolates | Microconidia | Macroconidia | |||
---|---|---|---|---|---|---|
Chain | Shape | Size | Shape | Size | ||
1 | FM3 | Present | Obovoid with flattened base, oval (0−1 septate), allantoid pyriform (rarely) | 10.7 µm× 9.14 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 64.63 µm × 15.43 µm |
2 | FM4 | Present | Obovoid with flattened base, pyriform, allantoid | 21.74 µm × 18.40 µm | Falcate to almost straight, slender, 1−4 septate (majority 3 septate) | 84.75 µm × 21.83 µm |
3 | FM5 | Present | Obovoid with flattened base, oval to long oval, elliptical, globose (rarely) | 9.30 µm × 7.94 µm | Falcate to straight, slender, 3−5 septate (majority 3 septate) | 70.10 µm × 16.89 µm |
4 | FM6 | Present | Obovoid with flattened base, oval to long oval, elliptical, globose (rarely) | 10.7 µm × 9.33 µm | Falcate to straight, slender, 3−5 septate (majority 3 septate) | 64.63 µm × 13.27 µm |
5 | FM7 | Present | Obovoid with flattened base, oval to long oval, elliptical, globose (rarely) | 9.30 µm × 8.45 µm | Falcate to straight, slender, 3−5 septate (majority 3 septate) | 70.13 µm × 15.43 µm |
6 | FM8 | Present | Obovoid with flattened base, oval to long oval, elliptical, globose (rarely) | 13.89 µm × 10.88 µm | Falcate to straight, slender, 3−5 septate (majority 3 septate) | 69.67 µm × 17.79 µm |
7 | FM9 | Present | Obovoid with flattened base, oval to long oval, elliptical, globose (rarely) | 13.83 µm × 12.83 µm | Falcate to straight, slender, 3−5 septate (majority 3 septate) | 88.16 µm × 12.5 µm |
8 | FM10 | Present | Obovoid with flattened base, oval to long oval, elliptical, globose (rarely) | 13.76 µm × 10.86 µm | Falcate to straight, slender, 3−5 septate (majority 3 septate) | 88.19 µm × 12.5 µm |
9 | FM11 | Present | Obovoid with flattened base, oval (0−1 septate), allantoid pyriform (rarely) | 13.59 µm × 12.96 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 72.03 µm × 12.50 µm |
10 | FM12 | Present | Obovoid with flattened base, oval to long oval, elliptical, globose (rarely) | 10.07 µm × 9.34 µm | Falcate to straight, slender, 3−5 septate (majority 3 septate) | 64.63 µm × 13.27 µm |
11 | FM13 | Present | Obovoid with flattened base, oval (0−1 septate), allantoid pyriform (rarely) | 6.47 µm × 6.47 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 57.35 µm × 18.98 µm |
12 | FS16 | Present | Obovoid with flattened base, oval (0−1 septate), allantoid pyriform (rarely) | 5.83 µm × 5.83 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 68.59 µm × 23.05 µm |
13 | FS18 | Present | Obovoid with flattened base, oval to long oval, elliptical, globose (rarely) | 4.49 µm × 3.24 µm | Falcate to straight, slender, 3−5 septate (majority 3 septate) | 56.09 µm × 11.29 µm |
14 | FS19 | Present | Obovoid with flattened base, oval (0−1 septate), allantoid pyriform (rarely) | 5.31 µm × 3.92 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 61.95 µm × 11.17 µm |
15 | FS20 | Present | Obovoid with flattened base, oval (0−1 septate), allantoid pyriform (rarely) | 5.58 µm × 5.58 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 69.19 µm × 12.16 µm |
16 | FS21 | Present | Obovoid with flattened base, oval (0−1 septate), allantoid pyriform (rarely) | 12.62 µm × 3.08 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 61.09 µm × 14.52 µm |
17 | FS22 | Present | Obovoid with flattened base, pyriform, allantoid | 11.62 µm × 3.25 µm | Falcate to almost straight, slender, 1−4 septate (majority 3 septate) | 245.98 µm × 23.82 µm |
18 | FS23 | Present | Obovoid with flattened base, oval to long oval, elliptical, globose (rarely) | 7.50 µm × 5.69 µm | Falcate to straight, slender, 3−5 septate (majority 3 septate) | 177.33 µm × 21.88 µm |
19 | FS24 | Present | Obovoid with flattened base, pyriform, allantoid | 6.90 µm × 4.53 µm | Falcate to almost straight, slender, 1−4 septate (majority 3 septate) | 127.5 µm × 26.64 µm |
20 | FS25 | Present | Obovoid with flattened base, pyriform, allantoid | 5.96 µm × 5.90 µm | Falcate to almost straight, slender, 1−4 septate (majority 3 septate) | 58.24 µm × 33.10 µm |
21 | FS26 | Present | Obovoid with flattened base, pyriform, allantoid | 6.41 µm × 4.54 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 61.29 µm × 19.03 µm |
22 | FS29 | Present | Obovoid with flattened base, pyriform, allantoid | 9.33 µm × 2.73 µm | Falcate to almost straight, slender, 1−4 septate (majority 3 septate) | 142.78 µm × 22.45 µm |
23 | FS31 | Present | Obovoid with flattened base, pyriform, allantoid | 2.65 µm × 2.65 µm | Falcate to almost straight, slender, 1−4 septate (majority 3 septate) | 127.51 µm × 24.40 µm |
24 | FS32 | Present | Obovoid with flattened base, pyriform, allantoid | 3.52 µm × 1.75 µm | Falcate to almost straight, slender, 1−4 septate (majority 3 septate) | 149.71 µm × 15.81 µm |
25 | FS34 | Present | Obovoid with flattened base, oval (0−1 septate), allantoid pyriform (rarely) | 7.14 µm × 1.55 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 94.29 µm × 17.27 µm |
26 | FD35 | Present | Obovoid with flattened base, oval (0−1 septate), allantoid pyriform (rarely) | 8.81 µm × 4.80 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 65.57 µm × 21.66 µm |
27 | FD41 | Present | Obovoid with flattened base, oval to long oval, elliptical, globose (rarely) | 9.54 µm × 4.78 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 54.68 µm × 18.00 µm |
28 | FD45 | Present | Obovoid with flattened base, pyriform, allantoid | 6.27 µm × 5.02 µm | Falcate to almost straight, slender, 1−4 septate (majority 3 septate) | 57.07 µm × 18.03 µm |
29 | FD47 | Present | Obovoid with flattened base, oval (0−1 septate), allantoid pyriform (rarely) | 9.58 µm × 4.58 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 118.51 µm × 15.54 µm |
30 | FD48 | Present | Obovoid with flattened base, oval (0−1 septate), allantoid pyriform (rarely) | 7.12 µm × 5.54 µm | Falcate to almost straight, slender, 1−3 septate (majority 3 septate) | 73.92 µm × 20.87 µm |
Sl. No. | Isolates of F. moniliforme | Shoot Length (cm) | No. of Chlorotic Leaves | Germination (%) |
---|---|---|---|---|
1 | FM3 | 21.25 d–g | 3.50 c–g | 67.86 a–c |
2 | FM4 | 21.00 d–h | 5.25 ab | 75.00 ab |
3 | FM5 | 22.75 b–e | 1.00 ij | 64.29 a–d |
4 | FM6 | 18.00 g–k | 1.75 hi | 67.86 a–c |
5 | FM7 | 21.75 c–f | 1.75 hi | 64.29 a–d |
6 | FM8 | 21.50 d-f | 3.00 d–h | 60.71 a–e |
7 | FM9 | 25.50 ab | 4.75 a–c | 50.00 b–g |
8 | FM10 | 26.50 a | 4.50 a–d | 39.29 d–g |
9 | FM11 | 25.00 a–c | 5.75 a | 60.71 a–e |
10 | FM12 | 20.50 e–i | 4.00 b–f | 75.00 ab |
11 | FM13 | 20.50 e–i | 3.00 d–h | 50.00 b–g |
12 | FS16 | 19.75 e–j | 2.25 g–i | 57.14 a–f |
13 | FS18 | 21.00 d–h | 3.25 c–h | 28.57 g |
14 | FS19 | 24.00 a–d | 4.25 a–e | 57.14 a–f |
15 | FS20 | 19.25 f–j | 1.00 ij | 35.71 e–g |
16 | FS21 | 18.00 g–k | 4.75 a–c | 53.57 b–g |
17 | FS22 | 21.75 c–f | 4.00 b–f | 46.43 c–g |
18 | FS23 | 19.25 f–j | 2.75 e–h | 35.71 e–g |
19 | FS24 | 21.75 c–f | 4.75 a–c | 57.14 a–f |
20 | FS25 | 19.25 f–j | 2.75 e–h | 32.14 fg |
21 | FS26 | 15.25 kl | 1.75 hi | 42.86 c–g |
22 | FS29 | 17.00 jk | 3.00 d–h | 39.29 d–g |
23 | FS31 | 12.50 l | 3.75 b–g | 46.43 c–g |
24 | FS32 | 18.00 g–k | 3.25 c–h | 39.29 d–g |
25 | FS34 | 18.50 f–k | 4.50 a–d | 32.14 fg |
26 | FD35 | 12.00 l | 2.50 f–i | 28.57 g |
27 | FD41 | 15.25 kl | 3.50 c–g | 60.71 a–e |
28 | FD45 | 17.75 h–k | 4.75 a–c | 57.14 a–f |
29 | FD47 | 17.25 i–k | 2.75 e–h | 32.14 fg |
30 | FD48 | 19.25 f–j | 3.50 c–g | 50.00 b–g |
31 | Control | 13.50 l | 0.00 j | 82.14 a |
CV (%) | 12.55 | 32.80 | 35.36 | |
Level of significance | * | * | * |
Sl. No | Isolates of B. subtilis | Radial Mycelial Growth (mm) | % Growth Inhibition over Control | ||
---|---|---|---|---|---|
9 DAI | 12 DAI | Mean | |||
1 | BS10 | 15.85 b | 18.89 c | 17.37 | 68.21 |
2 | BS17 | 18.77 b | 24.11 c | 21.44 | 60.76 |
3 | BS21 | 12.34 b | 18.67 c | 15.51 | 71.61 |
4 | BS26 | 14.52 b | 19.89 c | 17.21 | 68.50 |
5 | BS27 | 18.17 b | 24.33 c | 21.25 | 61.11 |
6 | BS31 | 39.75 a | 49.11 b | 44.43 | 18.69 |
7 | BS41 | 17.26 b | 20.45 c | 18.86 | 65.48 |
8 | BS8 | 17.23 b | 21.11 c | 19.17 | 64.92 |
9 | Control | 46.05 a | 63.22 a | 54.64 | - |
CV (%) | 18.37 | 20.07 | - | - | |
Level of significance | * | * | - | - |
Sl. No | Isolates of P. fluorescens | Radial Mycelial Growth (mm) | % Growth Inhibition over Control | ||
---|---|---|---|---|---|
9 DAI | 12 DAI | Mean | |||
1 | PF10 | 23.50 bc | 25.00 cd | 24.25 | 55.61 |
2 | PF11 | 49.27 a | 61.45 a | 55.36 | No inhibition |
3 | PF18 | 47.67 a | 56.67 a | 52.17 | 4.52 |
4 | PF2 | 39.62 a–c | 50.00 ab | 44.81 | 18 |
5 | PF5 | 38.78 a–c | 48.89 a–c | 43.84 | 19.77 |
6 | PF7 | 20.63 c | 24.56 d | 22.60 | 58.64 |
7 | PF8 | 21.01 c | 27.22 b–d | 24.12 | 55.86 |
8 | PF9 | 50.42 a | 65.11 a | 57.77 | No inhibition |
9 | Control | 46.05 ab | 63.22 a | 54.64 | - |
CV (%) | 35.65 | 30.07 | - | - | |
Level of significance | * | * | - | - |
Sl. No | Isolate of Achromobacter spp. | Radial Mycelial Growth (mm) | % Growth Inhibition over Control | ||
---|---|---|---|---|---|
9 DAI | 12 DAI | Mean | |||
1 | Achromobacter spp. | 12.57 b | 16.34 b | 14.46 | 73.54 |
2 | Control | 46.05 a | 63.22 a | 54.64 | - |
CV (%) | 11.80 | 9.58 | - | - | |
Level of significance | * | * | - | - |
Sl. No | Isolate of Trichoderma spp. | Radial Mycelial Growth (mm) | % Growth Inhibition over Control | ||
---|---|---|---|---|---|
9 DAI | 12 DAI | Mean | |||
1 | Trichoderma spp. | 14.07 b | 18.89 b | 16.48 | 69.84 |
2 | Control | 46.05 a | 63.22 a | 54.64 | - |
CV (%) | 9.23 | 2.97 | - | - | |
Level of significance | * | * | - | - |
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Al Amin, A.; Ali, M.H.; Islam, M.M.; Chakraborty, S.; Kabir, M.H.; Khokon, M.A.R. Variations in Morpho-Cultural Characteristics and Pathogenicity of Fusarium moniliforme of Bakanae Disease of Rice and Evaluation of In Vitro Growth Suppression Potential of Some Bioagents. Bacteria 2024, 3, 1-14. https://doi.org/10.3390/bacteria3010001
Al Amin A, Ali MH, Islam MM, Chakraborty S, Kabir MH, Khokon MAR. Variations in Morpho-Cultural Characteristics and Pathogenicity of Fusarium moniliforme of Bakanae Disease of Rice and Evaluation of In Vitro Growth Suppression Potential of Some Bioagents. Bacteria. 2024; 3(1):1-14. https://doi.org/10.3390/bacteria3010001
Chicago/Turabian StyleAl Amin, Abdullah, Md. Hosen Ali, Md. Morshedul Islam, Shila Chakraborty, Muhammad Humayun Kabir, and Md. Atiqur Rahman Khokon. 2024. "Variations in Morpho-Cultural Characteristics and Pathogenicity of Fusarium moniliforme of Bakanae Disease of Rice and Evaluation of In Vitro Growth Suppression Potential of Some Bioagents" Bacteria 3, no. 1: 1-14. https://doi.org/10.3390/bacteria3010001