An Integrated Approach to Control the Penetration Depth of 3D-Printed Hollow Microneedles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Device Design Printing and Assembly
- Design
- 2.
- 3D Printing
2.2.2. Inspection of 3D-Printed Parts
2.2.3. Skin Phantom Fabrication
2.2.4. Phantom Dimensional Verification
2.2.5. Profilometry
2.2.6. Mechanical Testing
- Base Substrate Characterization
- 2.
- Viscoelasticity Characterization
- 3.
- Indentation Parameters
2.2.7. Pycnometry
2.2.8. Simulation
2.2.9. Statistical Analysis
3. Results
3.1. Dimensional Inspection of 3D-Printed Parts
3.2. Skin Phantom Fabrication and Dimensional Verifcation
3.3. Profilometry
3.4. Mechanical Testing
- Substrate Characterization
- 2.
- Viscoelastic Characterization Using Microneedles
- 3.
- Microneedle Inspection Post-Indentation
3.5. Pycnometry
3.6. Validation of Puncture
3.7. Simulation
4. Discussion
4.1. Dimensional Inspection of 3D-Printed Parts
4.2. Profilometry
4.3. Mechanical Testing
4.4. Pycnometry
4.5. Simulation
4.6. Implications of the Research
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Disease/ Application | Cell Type | Microneedle Type | Material/ Fabrication | Penetration Depth | Citation |
---|---|---|---|---|---|
Melanoma tumors | Ovalbumin-pulsed dendritic cells | CryoMN | Cryogenic medium (PBS supplemented with 2.5% DMSO and 100 mM sucrose), molding | ~20 μm to 200 μm | [9] |
Cell transplantation | Human epidermal melanocytes, follicle dermal papilla cells, corneal keratocytes, corneal epithelial cells | HMN | Poly(methyl methacrylate) (PMMA), molding | 300 μm to 500 μm | [30] |
Heart regeneration after acute myocardial infarction (MI) | Cardiac stromal cells (CSCs) | Porous MN | Poly(vinyl alcohol) (PVA), micromolding | 400 μm to 500 μm | [31] |
Loading and intradermal delivery | Mesenchymal stem cells (MSCs), melanoctyes, antigen-pulsed dendritic cells | Porous MN | Methacrylated hyaluronic acid (MeHA), molding | ~50 μm to 200 μm | [32] |
Insulin delivery (diabetes mellitus) | Alginate-encapsulated pancreatic β-cells | Hydrogel MN | Hyaluronic acid (HA) matrix containing glucose-signal amplifiers (GSAs), micromolding | ~200 μm | [33] |
Material | Dimensions | Substrate | Index of Biomechanics | Citation |
---|---|---|---|---|
Thermoplastic | H = 700 μm Tip length = 150 μm Tip taper angle = 63.4° Reservoir depth = 180 μm Open channel = 30 μm * | Rabbit Skin | Bending force, dynamic loading tests and yield strength, axial compression testing of ~10 N for displacement of ~400 μm | [24] |
Stainless steel and poly(lactic-co-glycolic) acid (PLGA) | H = 600 μm Dbase = 300 μm Dbore = 90 μm | N/A ** | Buckling analysis for asymmetric hollow structures, failure of stainless steel HMN at 0.16 N and PLGA HMN at 0.19 N | [54] |
Polyvinyl alcohol | H = 600 μm Dbase = 200 μm Dtip = 30 μm Dbore = 25 μm | N/A ** | Axial and bending loading, force, and stress analysis, with bending force of 0.1788 N at the tip of HMN | [56] |
Clear resin V4 (acrylate based) | H = 600 μm Dbase = 1000 μm Dtip = 400 μm | N/A ** | Defective and clogged orifices | [57] |
Variable | Setting |
---|---|
d * (µm) | 800 |
H * (mm) | 3 |
Print Angle (°) | 0 |
Cleaning time (min) | 20 |
Curing time (min) | 10 |
h3 * (µm) | 150 |
AR * | 1.875 |
Runs | Amp (mm) | Rate (mm/s) | Delay (s) |
---|---|---|---|
A | 0.10 | 0.05 | 1.0 |
B | 0.15 | 0.10 | 1.5 |
C | 0.20 | 0.20 | 2.0 |
D | 0.25 | 0.50 | 2.5 |
Film | S (N/mm) | H (N/mm2) | δ (°) | E (Pa) | ν |
---|---|---|---|---|---|
1 | 900 | 31 | 11 | 41,000 | 0.39 |
2 | 1225 | 36.5 | 5 | 46,500 | 0.39 |
Run | F∞ (N) | Fmax (N) | R2 | Run | F∞ (N) | Fmax (N) | R2 |
---|---|---|---|---|---|---|---|
A | 0.0734 | 0.4851 | 0.9973 | AS | 0.2239 | 1.23 | 0.9967 |
B | 0.1233 | 1.2691 | 0.9934 | BS | 0.5514 | 3.648 | 0.9922 |
C | 0.1771 | 1.6807 | 0.9902 | CS | 0.461 | 3.1164 | 0.9962 |
D | 0.9256 | 2.8028 | 0.9832 | DS | 0.5627 | 4.4492 | 0.998 |
Run | E∞ (Pa) | Emax (Pa) | E1 (Pa) | E2 (Pa) | τ1 (s) | τ2 (s) |
---|---|---|---|---|---|---|
A | 4.04 × 104 | 2.67 × 105 | 1.23 × 105 | 7.87 × 104 | 1.9 | 25.6 |
B | 6.23 × 104 | 6.41 × 105 | 2.52 × 105 | 2.27 × 105 | 1.2 | 22.1 |
C | 8.24 × 104 | 7.82 × 105 | 3.70 × 105 | 2.07 × 105 | 0.6 | 15.3 |
D | 3.68 × 105 | 1.12 × 106 | 1.64 × 105 | 3.26 × 104 | 0.3 | 12.2 |
AS | 1.23 × 105 | 6.78 × 105 | 2.94 × 105 | 2.49 × 105 | 2.1 | 26.7 |
BS | 2.65 × 105 | 1.75 × 106 | 4.49 × 105 | 6.74 × 105 | 1.2 | 22.1 |
CS | 1.97 × 105 | 1.33 × 106 | 5.10 × 105 | 3.95 × 105 | 1.2 | 16.8 |
DS | 2.03 × 105 | 1.61 × 106 | 7.30 × 105 | 3.82 × 105 | 0.5 | 13.4 |
Run | HMNS Baseline ρ (g/cm3) | HMNS Post ρ (g/cm3) | p-Value (α = 0.05) | HMN Baseline ρ (g/cm3) | HMN Post ρ (g/cm3) | p-Value (α = 0.05) |
---|---|---|---|---|---|---|
BS/B | 1.4120 ± 0.0261 | 0.1691 ± 0.0010 | 9.69 × 10−5 | 1.4092 ± 0.0085 | 0.1500 ± 0.0017 | 6.08 × 10−6 |
Run | Actual Force (N) | Simulated Original Configuration (N) | Simulated Proposed Configuration (N) |
---|---|---|---|
A | 0.485 | 0.7 * | N/A ** |
B | 1.27 | 5.5 | N/A ** |
C | 1.68 | 2700 | N/A ** |
AS | 1.23 | 0.95 * | 1.52 |
BS | 3.65 | 0.28 | 1.75 |
CS | 3.12 | 0.6 | 1.85 |
Area | Key Findings | Recommendations |
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SLA and Dimensional Verification |
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Skin Phantom Fabrication and Biomechanical Tests |
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Puncture Characterization |
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Simulation |
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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Defelippi, K.M.; Kwong, A.Y.S.; Appleget, J.R.; Altay, R.; Matheny, M.B.; Dubus, M.M.; Eribes, L.M.; Mobed-Miremadi, M. An Integrated Approach to Control the Penetration Depth of 3D-Printed Hollow Microneedles. Appl. Mech. 2024, 5, 233-259. https://doi.org/10.3390/applmech5020015
Defelippi KM, Kwong AYS, Appleget JR, Altay R, Matheny MB, Dubus MM, Eribes LM, Mobed-Miremadi M. An Integrated Approach to Control the Penetration Depth of 3D-Printed Hollow Microneedles. Applied Mechanics. 2024; 5(2):233-259. https://doi.org/10.3390/applmech5020015
Chicago/Turabian StyleDefelippi, Kendall Marie, Allyson Yuuka Saumei Kwong, Julia Rose Appleget, Rana Altay, Maya Bree Matheny, Mary Margaret Dubus, Lily Marie Eribes, and Maryam Mobed-Miremadi. 2024. "An Integrated Approach to Control the Penetration Depth of 3D-Printed Hollow Microneedles" Applied Mechanics 5, no. 2: 233-259. https://doi.org/10.3390/applmech5020015