Viral Diseases in Aquaculture

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Aquatic Animals".

Deadline for manuscript submissions: 10 October 2024 | Viewed by 921

Special Issue Editor


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Guest Editor
Aquaculture Pathology Laboratory, School of Animal & Comparative Biomedical Sciences, The University of Arizona, Tucson, AZ 85721, USA
Interests: aquaculture; pathology; fish; shellfish; immunity

Special Issue Information

Dear Colleagues,

Aquaculture is the most profitable practice in the food production sector. However, diseases continue to pose a threat to the growth of aquaculture. Diseases in aquaculture are caused mainly by viruses, bacteria, fungi, and parasites. Particularly, viral diseases cause multibillion-dollar losses to the aquaculture industry, which has experienced more frequent viral disease outbreaks in the last two decades. According to the World Organization for Animal Health (OIE), the most listed pathogens in aquatic animals belong to viral families, accounting for more than 50% of OIE’s notifiable diseases for aquatic animals. Notably, OIE’s listed diseases for aquatic animals display only diseases causing mass mortality to cultured fish or shrimp. Some viruses that do not cause mass mortality do not catch the attention of aquaculture society, but they contribute to losses by stunting growth or increasing the susceptibility of the host.

Therefore, viral studies are necessary to give us a better understanding of viral diseases in aquaculture.

With that being said, we are pleased to invite you to submit your unpublished research to a Special Issue entitled “Viral Diseases in Aquaculture”. This Special Issue aims to present all aspects of viral diseases in aquaculture including molecular virology, viral evolution, pathogenesis, epidemiology, virus bioinformatics and viral immunology.

For this Special Issue, original research articles and reviews are welcome.

I/We look forward to receiving your contributions.

Dr. Hung N. Mai
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • molecular virology
  • viral evolution
  • pathogenesis
  • epidemiology
  • virus bioinformatics
  • viral immunology

Published Papers (1 paper)

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Research

14 pages, 3072 KiB  
Article
A New Cell Line from the Brain of Red Hybrid Tilapia (Oreochromis spp.) for Tilapia Lake Virus Propagation
by Aslah Mohamad, Matepiya Khemthong, Pirada Trongwongsa, Tuchakorn Lertwanakarn, Piyathip Setthawong and Win Surachetpong
Animals 2024, 14(11), 1522; https://doi.org/10.3390/ani14111522 - 22 May 2024
Viewed by 392
Abstract
Tilapia lake virus (TiLV) presents a substantial threat to global tilapia production. Despite the development of numerous cell lines for TiLV isolation and propagation, none have been specifically derived from red hybrid tilapia (Oreochromis spp.). In this study, we successfully established a [...] Read more.
Tilapia lake virus (TiLV) presents a substantial threat to global tilapia production. Despite the development of numerous cell lines for TiLV isolation and propagation, none have been specifically derived from red hybrid tilapia (Oreochromis spp.). In this study, we successfully established a new cell line, RHTiB, from the red hybrid tilapia brain. RHTiB cells were cultured for 1.5 years through over 50 passages and demonstrated optimal growth at 25 °C in Leibovitz-15 medium supplemented with 10% fetal bovine serum at pH 7.4. Morphologically, RHTiB cells displayed a fibroblast-like appearance, and cytochrome oxidase I gene sequencing confirmed their origin from Oreochromis spp. Mycoplasma contamination testing yielded negative results. The revival rate of the cells post-cryopreservation was observed to be between 75 and 80% after 30 days. Chromosomal analysis at the 25th passage revealed a diploid count of 22 pairs (2n = 44). While no visible cytopathic effects were observed, both immunofluorescence microscopy and RT-qPCR analysis demonstrated successful TiLV propagation in the RHTiB cell line, with a maximum TiLV concentration of 107.82 ± 0.22 viral copies/400 ng cDNA after 9 days of incubation. The establishment of this species-specific cell line represents a valuable advancement in the diagnostic and isolation tools for viral diseases potentially impacting red hybrid tilapia. Full article
(This article belongs to the Special Issue Viral Diseases in Aquaculture)
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