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Cells
Special Issues
The Applications of Flow Cytometry: Advances, Challenges, and Trends
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The Applications of Flow Cytometry: Advances, Challenges, and Trends

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Cell Methods".

Deadline for manuscript submissions: 31 May 2024 | Viewed by 4466

Special Issue Editor

Dr. David R. Kaplan

E-Mail Website
Guest Editor
Department of Pathology, Case Western Reserve University, 2854 Sedgewick Road, Shaker Heights, OH, USA
Interests: flow cytometry; signal amplification; cell-specific molecular expression levels; bipolar disorder; major depressive disorder; PTSD; multiple sclerosis; acute myocardial infarction
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Flow cytometry is a well-established and user-friendly single-cell technology that simultaneously measures multiple analyte expression patterns in individual cells. Since its development in the 1960s, it has enabled important analytical and clinical breakthroughs in immunology, cell biology, oncology, bacteriology, infectious disease, rheumatology, and molecular biology.

With its widespread application, flow cytometry has made remarkable progress. Recently, advancements in the field include technological innovations (i.e., spectral cytometry, mass cytometry, and imaging cytometry) and methodological innovations in acquisition and analysis. These advances have rendered flow cytometry an invaluable tool in studies of the immune system and other areas of cell biology.

This Special Issue will provide insights into the applications of using flow cytometry, covering the latest advances, current challenges, and future trends. It aims to broaden our understanding of basic research flow cytometry findings and potentially lead in translating new applications or new protocols into clinical strategies.

We would highly welcome the submission of original article, review, or communication on flow cytometry in research biology or medical science. Interested authors should consult the instructions using the following link: https://www.mdpi.com/journal/cells/instructions.

Dr. David R. Kaplan
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • flow cytometry
  • single cell
  • cell function
  • T-cell subset

Published Papers (1 paper)

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Review

34 pages, 2584 KiB  
Review
Advances and Challenges in Sepsis Management: Modern Tools and Future Directions
by Elena Santacroce, Miriam D’Angerio, Alin Liviu Ciobanu, Linda Masini, Domenico Lo Tartaro, Irene Coloretti, Stefano Busani, Ignacio Rubio, Marianna Meschiari, Erica Franceschini, Cristina Mussini, Massimo Girardis, Lara Gibellini, Andrea Cossarizza and Sara De Biasi
Cells 2024, 13(5), 439; https://doi.org/10.3390/cells13050439 - 2 Mar 2024
Cited by 2 | Viewed by 3951
Abstract
Sepsis, a critical condition marked by systemic inflammation, profoundly impacts both innate and adaptive immunity, often resulting in lymphopenia. This immune alteration can spare regulatory T cells (Tregs) but significantly affects other lymphocyte subsets, leading to diminished effector functions, altered cytokine profiles, and [...] Read more.
Sepsis, a critical condition marked by systemic inflammation, profoundly impacts both innate and adaptive immunity, often resulting in lymphopenia. This immune alteration can spare regulatory T cells (Tregs) but significantly affects other lymphocyte subsets, leading to diminished effector functions, altered cytokine profiles, and metabolic changes. The complexity of sepsis stems not only from its pathophysiology but also from the heterogeneity of patient responses, posing significant challenges in developing universally effective therapies. This review emphasizes the importance of phenotyping in sepsis to enhance patient-specific diagnostic and therapeutic strategies. Phenotyping immune cells, which categorizes patients based on clinical and immunological characteristics, is pivotal for tailoring treatment approaches. Flow cytometry emerges as a crucial tool in this endeavor, offering rapid, low cost and detailed analysis of immune cell populations and their functional states. Indeed, this technology facilitates the understanding of immune dysfunctions in sepsis and contributes to the identification of novel biomarkers. Our review underscores the potential of integrating flow cytometry with omics data, machine learning and clinical observations to refine sepsis management, highlighting the shift towards personalized medicine in critical care. This approach could lead to more precise interventions, improving outcomes in this heterogeneously affected patient population. Full article
(This article belongs to the Special Issue The Applications of Flow Cytometry: Advances, Challenges, and Trends)
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: Flow-cytometry evaluation of blood cells-bound surface FVIII in Hemophilia A and different coagulation states
Authors: Anjud Al-Mohannadi 1,4, Reem Yahia1, Hani Bibawi 2, Che-Ann Lachica 1, Watfa Osman1, Elkhansa Elbukhari Elgaali3, Anila Ejaz3, Mohammed Elanbari 1, Zainab Awada 1, Mohammed J Al-Kubaisi1, Muhammad Eln
Affiliation: 1 Research Department, Sidra Medicine, PO Box 26999, Doha, Qatar. 2 Pathology Department, Sidra Medicine, PO Box 26999, Doha, Qatar. 3 Hematology-Oncology Clinic, Sidra Medicine, PO Box 26999, Doha, Qatar. 4 College of Health and Life Sciences, Hamad Bin Khalifa University, Doha, Qatar.

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