Liquid Chromatography in the Analysis of Bioactive Compounds for Pharmaceutical Interest—3rd Edition

A special issue of Pharmaceuticals (ISSN 1424-8247). This special issue belongs to the section "Natural Products".

Deadline for manuscript submissions: 15 July 2024 | Viewed by 560

Special Issue Editors

Special Issue Information

Dear Colleagues,

Bioactive compounds, as natural primary and secondary metabolites, are used for the production of natural pharmaceuticals due to their pro-health properties. In order to confirm their identity, modern chromatographic techniques are used to allow for accurate quantitative and qualitative identification of the above-mentioned phytochemicals and their natural products. Liquid chromatography is one of the most efficient and robust specific techniques, due to the advantages of convenience and strong separation ability, as well as a wide range of material applications for identification. Liquid chromatography is widely used for the analysis of plants, nutraceuticals, pharmaceuticals, natural product quality control, or quantitative determination of bioactive compounds and others. The most commonly used methods for the identification of different plant materials and pharmaceuticals are ultra- and high-performance liquid chromatography with UV–VIS, fluorescence, and diode array, and are equipped with mass spectrometry or tandem mass spectrometry detection methods. It is important to mention that the latest scientific reports, also indicated in the 2nd edition of this Special Issue, indicate the significant analytical progress in pharmaceutical fields and that the application of liquid chromatography–mass spectrometry platforms for data acquisition is now common practice across the world. Therefore, for this Special Issue, we will publish studies concerning the latest scientific news, insights, and advances in the field of innovation of liquid chromatography for the analysis of bioactive compounds for pharmaceutical interest. Original research articles, short communications, and critical review articles dealing with the extraction and identification of bioactive compounds from natural matrices with biological and pharmaceutical interest are welcome. The information presented will arouse considerable interest among a large group of readers from different disciplines and research fields.

Prof. Dr. Jan Oszmianski
Dr. Sabina Lachowicz-Wiśniewska
Guest Editors

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant materials
  • pharmaceuticals
  • product quality
  • innovative methods
  • polyphenolic compounds
  • isoprenoids
  • bioactive compounds
  • food chemistry
  • natural products
  • mass spectrometry
  • extraction
  • LC-MS/MS techniques
  • HPLC-DAD-MS

Published Papers (1 paper)

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Research

20 pages, 1178 KiB  
Article
Simultaneous Analysis of Thirteen Compounds in Yeokwisan Using High-Performance Liquid Chromatography–Photodiode Array Detection and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry and Their Antioxidant Effects
by Chang-Seob Seo, So-Yeon Kim and Dong-Seon Kim
Pharmaceuticals 2024, 17(6), 727; https://doi.org/10.3390/ph17060727 - 4 Jun 2024
Viewed by 232
Abstract
Yeokwisan (YWS) is an herbal medicine prescription consisting of six oriental herbal medicines, developed to treat reflux esophagitis. We focused on developing an analytical method capable of simultaneously quantifying 13 compounds in YWS samples using high-performance liquid chromatography–photodiode array detection (HPLC–PDA) and ultra-performance [...] Read more.
Yeokwisan (YWS) is an herbal medicine prescription consisting of six oriental herbal medicines, developed to treat reflux esophagitis. We focused on developing an analytical method capable of simultaneously quantifying 13 compounds in YWS samples using high-performance liquid chromatography–photodiode array detection (HPLC–PDA) and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) and exploring their antioxidant effects. All compounds examined in both analytical systems were chromatographically separated on a SunFireTM C18 (4.6 × 250 mm, 5 μm) column and an Acquity UPLC BEH C18 (2.1 × 100 mm, 1.7 μm) column using gradient elution of a water–acetonitrile mobile phase. Antioxidant effects were evaluated based on radical scavenging activity (DPPH and ABTS tests) and ferrous ion chelating activity. In two analytical methods, the coefficient of determination of the regression equation was ≥0.9965, the recovery range was 81.11–108.21% (relative standard deviation (RSD) ≤ 9.33%), and the precision was RSD ≤ 11.10%. Application of the optimized analysis conditions gave quantitative analysis results for YWS samples of 0.02–100.36 mg/g. Evaluation of the antioxidant effects revealed that baicalein and baicalin exhibit significant antioxidant activity, suggesting that they play an important role in the antioxidant effects of YWS. Full article
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