Recent Scientific Developments in Forest Pathology

A special issue of Forests (ISSN 1999-4907). This special issue belongs to the section "Forest Health".

Deadline for manuscript submissions: 20 November 2024 | Viewed by 692

Special Issue Editor


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Guest Editor
Nature Research Centre, Laboratory of Plant Pathology, Akademijos St. 2, LT-08412 Vilnius, Lithuania
Interests: phytoplasma disease; molecular identification; classification; phylogeny; marker genes; forest plants; small fruit crops; insect vectors
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Special Issue Information

Dear Colleagues,

Forest plant ecosystems are economically important for the timber industry, food industry (wild berries), pharmacy (medicine plants), etc. Plant–pathogenic fungi, phytophthoras, bacteria (including phytoplasmas) and viruses can cause serious plant diseases, affecting natural ecosystems and plant biodiversity. These pathogenic microorganisms can be spread from wild forest plants to agricultural plants and vice versa via various mechanisms, including insect vectors and vegetative reproductions and propagations. Furthermore, global warming introduces insects and diseases to new geographic locations. Pathogenic microbes are identified via isolation in vitro and molecular tools (PGR, phylogenetic analysis, ELISA, etc.). Recent scientific developments include metagenomic microbiome investigations via next-generation throughput sequencing and bioinformatics. Furthermore, new environmentally friendly sustainable disease control methods are being developed, including biological control agents (BCAs). The most investigated pathogens are causing symptomatic diseases in agricultural and other economically important plants. However, many pathogens remain understudied, including uncultivable in vitro the Candidatus species in wild forests.

Potential topics include, but are not limited to:

  • Forest pathology: diseases of forest plants, including trees, shrubs, wild pome, stone, small fruit, nuts, medicine plants and other wild forest herbal plants;
  • Urban forests and diseases;
  • Forest entomology: insect vectors;
  • Diseases and forest ecology, management and restoration;
  • Diseases and climate change impacts, adaptation and mitigation in forests.

Dr. Deividas Valiunas
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Forests is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • identification of forest plant diseases and causing microbes
  • phylogenetic analysis of forest pathogens
  • plant microbiome and microbiota
  • forest trees, shrubs and fruit plants
  • forest medicine, herbal plants
  • insect vectors, biocontrol and disease management
  • climate change and diseases

Published Papers (1 paper)

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Research

13 pages, 7091 KiB  
Article
Molecular Detection of Phytophthora cinnamomi by RPA-CRISPR/Cas12a-Mediated Isothermal Amplification
by Xiaoqiao Xu, Tingting Dai, Qin Xiong, Jing Yang, Jiahui Zang and Tingli Liu
Forests 2024, 15(5), 772; https://doi.org/10.3390/f15050772 - 28 Apr 2024
Viewed by 506
Abstract
Background: Phytophthora cinnamomi is one of the soil-borne pathogens that causes root rot and stem rot in many plants globally. P. cinnamomi has serious economic, social, and environmental impacts, threatening natural ecosystems and biodiversity. Methods: In this study, a molecular detection method based [...] Read more.
Background: Phytophthora cinnamomi is one of the soil-borne pathogens that causes root rot and stem rot in many plants globally. P. cinnamomi has serious economic, social, and environmental impacts, threatening natural ecosystems and biodiversity. Methods: In this study, a molecular detection method based on Recombinant polymorphic amplification (RPA) combined using the CRISPR/Cas12a system was developed for P. cinnamomi. The method was found to be highly specific for P. cinnamomi. Results: The results showed that 10 P. cinnamomi isolates were positive; however, 21 Phytophthora species, 4 Phytopythium species, 18 fungal species, and 2 Bursaphelenchus species were negative. In total, 10 pg·µL−1 of P. cinnamomi genomic DNA can be detected. The detection process is performed within 20 min at 37 °C, which makes it fast and convenient for use. Discussion: In conclusion, the RPA-CRISPR/Cas12a system in this study is a promising tool for the rapid and sensitive detection of P. cinnamomi in plant samples. Full article
(This article belongs to the Special Issue Recent Scientific Developments in Forest Pathology)
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