Measurement and Standardization in Microfluidic Devices: Fabrication and Testing

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "E:Engineering and Technology".

Deadline for manuscript submissions: 30 June 2024 | Viewed by 597

Special Issue Editors


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Guest Editor
Metrology Department, Instituto Português da Qualidade (IPQ), 2829-513 Caparica, Portugal
Interests: metrology; standardization; measurements; volume and flow

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Guest Editor
National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Interests: microfluidics; biomedical engineering; cell biology; cell imaging; tissue engineering
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Guest Editor
Division of Applied Mechanics, Office of Science and Engineering Laboratories, Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD 20993, USA
Interests: bioaerosols; medical devices; microfluidics; medical countermeasures
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In the last decade, the field of microfluidics has seen a phenomenal growth. However, in absence of well-established calibration procedures or standards, high quality microfluidic device production has been limited to experienced manufacturer who possess relevant expertise. Despite the academic impact of microfluidics (societal, health, well-being, environment), success stories are rare in comparison. There appears to be a gap between laboratory developed microfluidic devices and a reliable and reproducible product because of several reasons - lack of reliability in microfluidic components, lack of standardized methodologies, existence of a variety of connectors but no consensus on testing needed to evaluate them, incompatibility of materials chosen during device ideation in context of scaling up, lack of traceability of the physical and chemical functionality of the device, and for metrological operations involved in the lifetime of microfluidic devices. To help address this gap, this special issue is soliciting articles that can be adapted as guidelines, methodologies, etc. by standard development organizations and used in future designing, material selection and testing of microfluidic devices. We hope the articles written to address the gap will not only de-risk the microfluidics technology and lead to more reliable devices, and in addition may also enable small and large businesses to streamline their product development in a cost-effective manner.

Dr. Elsa Batista
Dr. Darwin R. Reyes
Dr. Suvajyoti Guha
Guest Editors

Manuscript Submission Information

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Keywords

  • microfluidics
  • metrology
  • standardization
  • measurement
  • traceability
  • fabrication

Published Papers (1 paper)

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Research

15 pages, 3584 KiB  
Article
Mechanical Characterization of the Erythrocyte Membrane Using a Capacitor-Based Technique
by Doriana Dorta, Carlos Plazaola, Jafeth Carrasco, Maria F. Alves-Rosa, Lorena M. Coronado, Ricardo Correa, Maytee Zambrano, Braulio Gutiérrez-Medina, Erick Sarmiento-Gómez, Carmenza Spadafora and Guadalupe Gonzalez
Micromachines 2024, 15(5), 590; https://doi.org/10.3390/mi15050590 - 28 Apr 2024
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Abstract
Pathological processes often change the mechanical properties of cells. Increased rigidity could be a marker of cellular malfunction. Erythrocytes are a type of cell that deforms to squeeze through tiny capillaries; changes in their rigidity can dramatically affect their functionality. Furthermore, differences in [...] Read more.
Pathological processes often change the mechanical properties of cells. Increased rigidity could be a marker of cellular malfunction. Erythrocytes are a type of cell that deforms to squeeze through tiny capillaries; changes in their rigidity can dramatically affect their functionality. Furthermore, differences in the homeostatic elasticity of the cell can be used as a tool for diagnosis and even for choosing the adequate treatment for some illnesses. More accurate types of equipment needed to study biomechanical phenomena at the single-cell level are very costly and thus out of reach for many laboratories around the world. This study presents a simple and low-cost technique to study the rigidity of red blood cells (RBCs) through the application of electric fields in a hand-made microfluidic chamber that uses a capacitor principle. As RBCs are deformed with the application of voltage, cells are observed under a light microscope. From mechanical force vs. deformation data, the elastic constant of the cells is determined. The results obtained with the capacitor-based method were compared with those obtained using optical tweezers, finding good agreement. In addition, P. falciparum-infected erythrocytes were tested with the electric field applicator. Our technique provides a simple means of testing the mechanical properties of individual cells. Full article
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